Author： Hardie L.J. Rayner D.V. Holmes S. Trayhurn P.
Publisher： Elsevier
ISSN： 0006-291X
Source： Biochemical and Biophysical Research Communications, Vol.223, Iss.3, 1996-06, pp. : 660-665
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Abstract
The development of a sensitive enzyme-linked immunosorbent assay (ELISA) is described for the quantitation of plasma leptin levels in both mice and rats. Approximately 1.5ng leptin/ml plasma was detected in lean mice but significantly (p<0.01) less was found in the plasma of ob/ob mice. In lean Zucker rats leptin circulated at approximately 4ng/ml plasma whereas levels were elevated more than 6-fold in fa/fa rats. Circulating leptin levels declined (p<0.05) in lean rats which were fasted or exposed to 4#°C for 24h, but subsequently recovered to normal within 12h of refeeding or warming, respectively. Administration of insulin increased leptin levels in lean rats within 4h (p<0.01). However, leptin levels were unchanged in fa/fa rats exposed to the same physiological stimuli.
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