Direct Interaction of the Mitochondrial Membrane Protein Carnitine Palmitoyltransferase I with Bcl-2

Author: Paumen M.B.   Ishida Y.   Han H.   Muramatsu M.   Eguchi Y.   Tsujimoto Y.   Honjo T.  

Publisher: Elsevier

ISSN: 0006-291X

Source: Biochemical and Biophysical Research Communications, Vol.231, Iss.3, 1997-02, pp. : 523-525

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Abstract

To demonstrate interaction between the mitochondrial membrane proteins CPT I and Bcl-2 the yeast two-hybrid system was used. Full-length CPT I was required for binding to Bcl-2. Direct protein interaction was confirmed in a GST binding assay and in co-immunoprecipitations using two different kinds of anti-Bcl-2 antibodies.

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