Author： Muraki K. Imaizumi Y. Ohya S. Sato K. Takii T. Onozaki K. Watanabe M.
Publisher： Elsevier
ISSN： 0006-291X
Source： Biochemical and Biophysical Research Communications, Vol.236, Iss.2, 1997-07, pp. : 340-343
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Abstract
Vascular endothelial cells have several types of Ca2+-dependent K+current (IK-Ca). Here, we describe apamin-sensitive IK-Cawhich is activated by treatment with histamine (His) in human umbilical vein endothelial cells (HUVECs). In 65 % of HUVECs examined, 100 nM apamin potently inhibited IK-Caand hyperpolarization induced by His (19 and 7 % of control, respectively). In contrast, application of 5 mM tetraethylammonium, a non-selective K channel blocker, or 100 nM iberiotoxin, a selective K channel blocker for a large conductance Ca2+-dependent K+channel, had small (78 % of control) or no effects (102 % of control) on IK-Ca, respectively. These findings suggest that apamin-sensitive Ca2+-dependent K+channels are expressed in HUVECs and activated by receptor stimulation.
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