1,25-Dihydroxyvitamin D₃Induced Activation and Subsequent Nuclear Translocation of MAPK Is Upstream Regulated by PKC in HL-60 Cells

Author： Marcinkowska E. Więdłocha A. Radzikowski C.

Publisher： Elsevier

ISSN： 0006-291X

Source： Biochemical and Biophysical Research Communications, Vol.241, Iss.2, 1997-12, pp. : 419-426

Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.

Previous Menu Next

Abstract

1,25-Dihydroxyvitamin D₃(1,25(OH)₂D₃) in addition to its classical role in calcium homeostasis regulates cell differentiation. The mechanisms involved in mediating numerous functions of 1,25(OH)₂D₃are not clearly understood. In addition to genomic actions involving nuclear vitamin D receptor (VDR), some rapid nongenomic responses have been observed, but the full signalling pathway activated by 1,25(OH)₂D₃has still not been described. Our recent data allow for better understanding of nongenomic effects evoked by 1,25(OH)₂D₃. In this paper we show that mitogen activated protein kinase (MAPK) is activated in HL-60 promyelocytic leukemia cells and in normal human keratinocytes under exposure to differentiation inducing concentrations of 1,25(OH)₂D₃. The MAPK is then transported to the cell nucleus in active form, which is different from the activation evoked by fetal calf serum. Experiments utilising tyrosine kinase inhibitor suggested that the postulated putative membrane vitamin D receptor, if it exists, does not have tyrosine kinase activity. Usage of protein kinase C (PKC) inhibitor allowed to state that PKC is an upstream element in the MAPK signalling pathway.

Related content

1,25-Dihydroxyvitamin D₃ Suppresses the Expression of the VCAM-1 Receptor, VLA-4 in Human Leukemic HL-60 Cells

By Kaneko A. Suzuki S. Hara M. Mori J-I. Kumagai M. Yajima H. Yamashita K. Kakizawa T. Yamazaki M. Takeda T. Miyamoto T. Ichikawa K. Hashizume K.

Biochemical and Biophysical Research Communications, Vol. 255, Iss. 2, 1999-02 ,pp. : 371-376 (6)

Elsevier

Access to resources Recommend Favorite

Activation of PKC but Not of ERK Is Required for Vitamin E-Succinate-Induced Apoptosis of HL-60 Cells

By Bang O-S. Park J-H. Kang S-S.

Biochemical and Biophysical Research Communications, Vol. 288, Iss. 4, 2001-11 ,pp. : 789-797 (9)

Elsevier

Access to resources Recommend Favorite

1,25-Dihydroxyvitamin D₃ Targets PKC-&bgr;II but Not PKC-&agr; to the Basolateral Plasma Membranes of Rat Colonocytes

By Wali R.K. Bissonnette M. Skarosi S. Khare S. Sitrin M. Brasitus T.A.

Biochemical and Biophysical Research Communications, Vol. 250, Iss. 1, 1998-09 ,pp. : 48-52 (5)

Elsevier

Access to resources Recommend Favorite

Antagonistic Action of Novel 1α,25-Dihydroxyvitamin D₃-26,23-Lactone Analogs on 25-Hydroxyvitamin-D₃-24-hydroxylase Gene Expression Induced by 1α,25-Dihydroxy-vitamin D₃ in Human Promyelocytic Leukemia (HL-60) Cells

By Ishizuka S. Miura D. Eguchi H. Ozono K. Chokki M. Kamimura T. Norman A.W.

Archives of Biochemistry and Biophysics, Vol. 380, Iss. 1, 2000-08 ,pp. : 92-102 (11)

Elsevier

Access to resources Recommend Favorite

CREB DNA binding activation by a 50-Hz magnetic field in HL60 cells is dependent on extra- and intracellular Ca²⁺ but not PKA, PKC, ERK, or p38 MAPK

By Zhou J. Yao G. Zhang J. Chang Z.

Biochemical and Biophysical Research Communications, Vol. 296, Iss. 4, 2002-08 ,pp. : 1013-1018 (6)

Elsevier

Access to resources Recommend Favorite