Mycobacterium tuberculosis KatG Is a Peroxynitritase

Author： Wengenack N.L.   Jensen M.P.   Rusnak F.   Stern M.K.  

Publisher： Elsevier

ISSN： 0006-291X

Source： Biochemical and Biophysical Research Communications, Vol.256, Iss.3, 1999-03, pp. : 485-487

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Abstract

Mycobacterium tuberculosis resides within the highly oxidative environment of the human macrophage and previous reports have indicated that these mycobacteria are susceptible to reactive nitrogen intermediates including peroxynitrite. This work provides evidence that the Mycobacterium tuberculosis hemoprotein KatG acts as an efficient peroxynitritase exhibiting a kapp of 1.4 × 105 M-1s-1 for peroxynitrite decomposition at pH 7.4 and 37°C. The ability of KatG to act as a peroxynitritase adds to its growing list of enzymatic activities and may in part explain the ability of Mycobacterium tuberculosis to persist in macrophages.

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