Author： Vier J. Linsinger G. Häcker G.
Publisher： Elsevier
ISSN： 0006-291X
Source： Biochemical and Biophysical Research Communications, Vol.261, Iss.1, 1999-07, pp. : 71-78
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
Cytochrome c is thought to play an important role in the initiation of apoptosis following its release from mitochondria. It is controversial whether such release is also involved in caspase activation and apoptotic cell death after ligation of the cell surface molecule Fas. We addressed this issue by investigating cells from the human cell lines Jurkat and SKW6 which had been treated with the inhibitor of the mitochondrial F0/F1-ATPase, oligomycin. Oligomycin-treatment led, over a wide range of concentrations, to ATP-depletion and, at similar concentrations, abrogated the appearance of caspase-3-like activity caused by stauroporine. Electroporation of cytochrome c protein into intact cells induced caspase activation in both cell lines and significant nuclear apoptosis in Jurkat cells. In ATP-depleted cells, electroporation of cytochrome c induced neither caspase activation nor nuclear fragmentation. Fas-induced caspase activation and nuclear apoptosis, however, were unaffected by the depletion of ATP. Thus, cytochrome c is unlikely to be an important factor in Fas-induced cell death.
Related content
Access to resources
Recommend
By Varnes M.E. Chiu S-M. Xue L-Y. Oleinick N.L.
Biochemical and Biophysical Research Communications, Vol. 255, Iss. 3, 1999-02 ,pp. :
By Ruemmele F.M. Beaulieu J.F. O'Connell J. Bennett M.W. Seidman E.G. Lentze M.J.
Biochemical and Biophysical Research Communications, Vol. 290, Iss. 4, 2002-02 ,pp. :