Author： Nishigaki T. Zamudio F.Z. Possani L.D. Darszon A.
Publisher： Elsevier
ISSN： 0006-291X
Source： Biochemical and Biophysical Research Communications, Vol.284, Iss.2, 2001-06, pp. : 531-535
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Abstract
Speract, a decapeptide from sea urchin egg jelly, induces various sperm responses. Stopped-flow fluorometry was used to examine the binding of labeled speract and the intracellular changes in pH (pHi) and Ca2+ ([Ca2+]i) it induces in sperm. We observed significant time delays for the increase in pHi and [Ca2+]i induced by 200 nM speract (69 and 190 ms, respectively). Also, we found that the receptor undergoes a pHi-dependent affinity change at around 129 ms. These time delays probably reflect biochemical processes underlying each sperm response to speract that circumscribe the time sequence of the signaling events.
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