Flowering Plant Sperm Contains a Cytosolic Soluble Protein Factor Which Can Trigger Calcium Oscillations in Mouse Eggs

Publisher： Elsevier

ISSN： 0006-291X

Source： Biochemical and Biophysical Research Communications, Vol.287, Iss.1, 2001-09, pp. : 56-59

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Abstract

There is evidence showing that the sperm-induced Ca²⁺ oscillations in mammalian eggs at fertilization are triggered by a sperm-derived protein factor. It was established recently that the activity of the putative sperm protein in causing Ca²⁺ oscillations in mammalian eggs is not species-specific in vertebrates (1, 16). Here we report that cytosolic soluble extracts derived from flowering plant sperms in Brassica campestris can also induce fertilization-like Ca²⁺ oscillations when microinjected into mouse eggs. The factor responsible for inducing Ca²⁺ oscillations in the plant sperm was sperm-specific and heat- or trypsin-labile. Eight to ten sperm equivalents of the plant sperm extracts had enough activity to trigger Ca²⁺ oscillations in mouse eggs. Our study suggests that, although plant and mammal are evolutionary divergent species, the activity of the putative sperm protein factor in triggering Ca²⁺ signaling in mammalian eggs is not specific to the animal kingdom.