Cobalt ions influence proliferation and function of human osteoblast-like cells

Author： Anissian Lucas Stark André Dahlstrand Henrik Granberg Barbro Good Victoria Bucht Elisabet

ISSN： 0001-6470

Source： Acta Orthopaedica Scandinavica, Vol.73, Iss.3, 2002-06, pp. : 369-374

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Abstract

Cobalt is the major component in many orthopedic implants and the introduction of a second generation of metal on metal bearing prosthesis systems actualizes the toxicity and biocompatibility of this compound. We studied the effect of cobalt ions on primary cultures of human osteoblast-like cells. Cobalt ions dissolved in cell culture medium caused a dose-dependent decrease in proliferation of human osteoblasts measured as (³H)thymidine incorporation. We also found that cobalt ion-enriched medium increased the production of interleukin-6 from the osteoblast-like cells. Furthermore, incubation of osteoblasts with cobalt ion-enriched medium reduced collagen type I and osteocalcin production in a dose-dependent manner when 1,25 dihydroxyvitamin-D₃ was added to the culture medium. Cobalt concentrations below 10 µg/mL or 0.17 mmol/mL in the cell culture medium had no significant effect on human osteoblast proliferation and function.