Author: Jang Am Okabe Satoshi Watanabe Yoshimasa Kim In S Bishop Paul L
Publisher: NRC Research Press
ISSN: 1496-256X
Source: Journal of Environmental Engineering and Science, Vol.4, Iss.5, 2005-09, pp. : 413-420
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
Optimization of the nitrification processes in biofilms is important for effective nitrogen removal because nitrification in an aerobic biofilm is considered to be a less than reliable process. Thus, one of the main factors to improve biological nitrogen removal processes is a better understanding of the microbiology and population dynamics of ammonia oxidizing bacteria (AOB) in wastewater treatment biofilms. Although the AOB in wastewater treatment have been qualitatively and quantitatively studied, information on their actual populations and activities is still limited. Therefore, the areal cell density of AOB in domestic wastewater biofilms on a partially submerged rotating biological contactor (RBC) was determined by fluorescent in situ hybridization (FISH) with a set of 16S rRNA-targeted oligonucleotide probes. The growth kinetics of the in situ AOB was also studied. Although low numbers of AOB were found at the deeper layers where oxygen was depleted, they were primarily detected in the upper and middle layers of the biofilm. The maximum specific growth rate (
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