Caffeine Sensitivity of the Yeast Saccharomyces cerevisiae with Mutant MCD4 Is Associated with Disturbances of Calcium Homeostasis and Degradation of Misfolded Proteins

Author： Fominov G.   Ter-Avanesyan M.  

Publisher： MAIK Nauka/Interperiodica

ISSN： 0026-8933

Source： Molecular Biology, Vol.39, Iss.3, 2005-05, pp. : 410-420

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Abstract

MCD4 codes for a protein involved in glycosylphosphatidylinositol synthesis in the yeast Saccharomyces cerevisiae. Some MCD4 mutations have effects potentially unrelated to defects in the synthesis of phospholipids of this group. The ssu21 mutation of MCD4 causes caffeine sensitivity. To study the molecular basis of this phenotype, yeast genes were screened for multicopy suppressors of caffeine sensitivity of the ssu21 mutant. The screening revealed genes involved in aminoglycerophospholipid metabolism, protein degradation, and the unfolded protein response. The suppressor effect of the cloned genes increased at a higher concentration of extracellular calcium. Caffeine sensitivity of the ssu21 mutant appeared to be associated with cytoplasmic accumulation of misfolded proteins.

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