Functional interaction of the RNA polymerase II Rpb11 subunit with the subunit Med18 (Srb5) of the Saccharomyces cerevisiae mediator complex

Author： Proshkina G. Shpakovskii G.

Publisher： MAIK Nauka/Interperiodica

ISSN： 1068-1620

Source： Russian Journal of Bioorganic Chemistry, Vol.35, Iss.4, 2009-07, pp. : 518-522

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Abstract

The SRB5 gene encoding the Med18 (Srb5) subunit of the Mediator complex of the Saccharomyces cerevisiae transcription apparatus was identified as a multicopy suppressor of the Leu111Ala (L111A) point mutation in the C-terminal region of the yeast RNA polymerase II subunit Rpb11. Thus, the functional interaction between one of the Mediator components and the core of the major transcription enzyme was shown for the first time. It is also important that the suppressed point mutation is located in the short C-terminal region of the Rpb11 subunit, which, as was demonstrated in our previous studies, has played an essential role in the evolution of the eukaryotic transcription machinery.