Enhancement of specific cellular immune response induced by DNA vaccines encoding PML-RARα and hIL-2 genes

Author： Cen Dongzhi   Hu Gang   Zhou Yubing   Yang Lijian   Chen Shaohua   Schmidt Christian A.   Li Yangqiu  

Publisher： Maney Publishing

ISSN： 1024-5332

Source： Hematology, Vol.15, Iss.2, 2010-04, pp. : 88-95

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Abstract

A DNA vaccine encoding PML-RAR α fusion gene is thought to be a promising approach for acute promyelocytic leukemia patients to enhance immune responses after attaining complete remission. In this study, we sought to enhance cellular immunity by coexpressing human interleukin (hIL)-2 genes. Successfully constructed plasmids PML-RAR α-hIL-2-pIRES, PML-RAR α-pIRES and hIL-2-pIRES were delivered intramuscularly in BALB/C mice at 14-day intervals for three cycles. The cellular immune responses with respect to the specific cytotoxicity of spleen cells; interferon-γ secretion in sera, and the T-cell receptor rearrangement excision circles of thymocyte were significantly increased from PML-RARα-hIL-2-pIRES immunized mice. Our results indicate that a DNA vaccine with PML fusion gene segment and hIL-2 together might elicit increased cellular immune responses in mice.

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