The effect of a short heat treatment on the in vitro induced androgenesis in Silene latifolia ssp. alba

Author： Šafářová D.   Kopecký D.   Vagera J.  

Publisher： Springer Publishing Company

ISSN： 0006-3134

Source： Biologia Plantarum, Vol.49, Iss.2, 2005-06, pp. : 261-264

Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.

Previous Menu Next

Abstract

The effect of a short heat treatment in combination with different culture medium composition on the efficiency of in vitro induced androgenesis in Silene latifolia ssp. alba was studied. The heat shocks (33 and 37°C) were applied for 1, 3, and 5 d. The best androgenic response was observed at 25°C and after a one-day treatment at 33°C. All other treatments reduced androgenic response. Among different media compositions tested, the most satisfactory results were obtained on BMS medium supplemented with 6-benzylaminopurine (0.5 mg dm−3) and sucrose. The green, albino and chimeric, only female, plants were regenerated. Flow cytometry of 110 regenerants identified haploids, mixoploids (n+2n and 2n+4n) and dihaploids.

Related content

Characterization of two SEPALLATA MADS-box genes from the dioecious plant Silene latifolia

By Matsunaga Sachihiro   Uchida Wakana   Kejnovsky Eduard   Isono Erika   Moneger Francoise   Vyskot Boris   Kawano Shigeyuki  

Sexual Plant Reproduction, Vol. 17, Iss. 4, 2004-12 ,pp. : 189-193 (5)

Springer Publishing Company

Access to resources Recommend Favorite

On the application of the Linnaean names Cucubalus reflexus, Silene nocturna and Silene mutabilis (Caryophyllaceae)

By Peruzzi Lorenzo   Jarvis Charles E.   Carta Angelino  

Taxon, Vol. 63, Iss. 3, 2014-06 ,pp. : 651-652 (2)

International Association for Plant Taxonomy

Access to resources Recommend Favorite

In vitro propagation of Artemisia petrosa ssp. eriantha: potential for the preservation of an endangered species

By Pace L   Pacioni G   Spano L  

Plant Biosystems, Vol. 138, Iss. 3, 2004-11 ,pp. : 291-294 (4)

Taylor & Francis Ltd

Access to resources Recommend Favorite

Gender-specific flowering responses to day length in the dioecious plant Silene latifolia at different temperatures

By Wang Xianzhong  

Sexual Plant Reproduction, Vol. 20, Iss. 1, 2007-03 ,pp. : 45-50 (6)

Springer Publishing Company

Access to resources Recommend Favorite

Phylogenetic Relationships of Silene multinervia and Silene Section Conoimorpha (Caryophyllaceae)

By Rautenberg Anja.   Sloan Daniel B.   Aldén Vivian.   Oxelman Bengt.  

Systematic Botany, Vol. 37, Iss. 1, 2012-03 ,pp. : 226-237 (12)

American Society of Plant Taxonomists

Access to resources Recommend Favorite