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Comparative study of postradiation genotoxic changes in mammalian cells by biochemical and cytogenetic methods

Author： Ivanov S. Yashmanov V. Kovan’ko E. Vorobtsova I. Poroshina T. Bershtein L.

Publisher： Springer Publishing Company

ISSN： 0007-4888

Source： Bulletin of Experimental Biology and Medicine, Vol.142, Iss.6, 2006-12, pp. : 679-682

Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.

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Abstract

The results of studying the genotoxic effect of radiation on endometrial cells of irradiated rats using comet assay correlated with the index of spontaneous DNA degradation. Studying the genotoxic effect of in vitro irradiation (2 Gy) on human lymphocytes by means of chromosome analysis and fluorometric assay of nucleoids DNA in irradiated leukocytes also yielded similar results. Measuring of the biochemical marker holds much promise as an express method of evaluation of the genotoxic effect of radiation on mammalian somatic cells.

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