Interspecific Polymorphism of Gene lipL32 Restriction Profiles of Pathogenic Leptospires

Author: Vaganova A.   Freilikhman O.   Stoyanova N.   Tokarevich N.  

Publisher: Springer Publishing Company

ISSN: 0007-4888

Source: Bulletin of Experimental Biology and Medicine, Vol.149, Iss.5, 2010-10, pp. : 609-611

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Abstract

We analyzed restriction fragment length polymorphism of lipL32 gene encoding outer membrane protein in three leprospira genomic species (L. interrogans, L. kirschneri, and L. borgpetersenii) using Bsa29 I and Bam HI restriction endonucleases. It was found that restriction profiles of the studied gene were similar at both the intraspecific and serogroup levels; variability was revealed only at the interspecific level, which can be explained by relatively low variability of lipL32 gene.

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