Differentiation of the high molecular weight glutenin subunit Dt x2.1 of Aegilops tauschii indicated by partial sequences of its encoding gene and SSR markers
Author：
Lu C.
Yang W.
Lu B.
Publisher：
Springer Publishing Company
ISSN：
0014-2336
Source：
Euphytica,
Vol.141,
Iss.1-2, 2005-01,
pp. : 75-83
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Abstract
The high molecular weight glutenin subunits (HMW-GS) are important components of storage proteins in common wheat (Triticum aestivum L.). Conventional techniques for identifying the HMW-GS apply SDS-PAGE. To study differentiation of the HMW glutenin genes, partial DNA sequences of the concerned alleles were characterized from eight accessions of hexaploid synthetic wheat and common wheat varieties, and compared with the available sequences. SSR markers based on, and generated from, DNA sequences of the D genome of common wheat and Aegilops tauschii were analyzed to estimate genetic relationships of the accessions. Results showed that the HMW-GS Dtx2.1 determined by SDS-PAGE could be identified as two types at the DNA sequence level. One type, designated as Dt x2.1a, showed identical SNPs to the Dt x 1.5 allele and another type, designated as Dt x2.1b, had different characteristics. The SSR analysis revealed a relatively close genetic relationship of the two accessions containing the Dt x2.1a allele to the accessions containing the Dt x1.5 allele. It was concluded that the conventional SDS-PAGE has limitations in determining some HMW-GS, because the minor changes on the DNA sequence of a particular glutenin subunit cannot be detected at protein level, and that the AS-PCR method based on variation of DNA sequences of particular genes will provide a more powerful tool for identifying the HMW-GS. The differentiation of the genes encoding the HMW-GS is probably more rapid than expected. The close relationship between evolutionary changes of the microsatellite DNA and the HMW-GS genes detected in this study needs to be further investigated.
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