(T_2AG_3)_n Telomeric Sequence Hybridization Indicating Centric Fusion Rearrangements in the Karyotype of the Rodent Oryzomys Subflavus

Author: Andrades-Miranda J.   Zanchin N.I.T.   Oliveira L.F.B.   Langguth A.R.   Mattevi M.S.  

Publisher: Springer Publishing Company

ISSN: 0016-6707

Source: Genetica, Vol.114, Iss.1, 2002-01, pp. : 11-16

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Abstract

Chromosome preparations of 30 specimens of Oryzomys subflavus trapped in eight Brazilian localities were C-, and G-banded and analyzed by fluorescence in situ hybridization (FISH). Two karyotypes were found, 2n = 50/FN = 64, at three coastal localities of the Atlantic Forest domain, and 2n = 58/FN = 70 at two sites located in the Cerrado biome, Brazil Central. Two fluorescence in situ hybridization (FISH) patterns of the telomeric sequence (T_2AG_3)_n were observed: in both karyotypes the probes hybridized to the telomeres of all chromosomes and also a hybridization signal in the centromeric regions of two autosome pairs was seen in the 2n = 50 karyotype. These results, together with the occurrence of other diploid numbers described in the literature, suggest that O. subflavus is a complex species, bearing fusion/fission rearrangements proper to the different biomes which it inhabits.

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