Estimation of the Temporal Course of Evoked Secretion of Transmitter Quanta in the Frog Neuromuscular Junction

ISSN： 0090-2977

Source： Neurophysiology, Vol.33, Iss.2, 2001-03, pp. : 69-78

Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.

Previous Menu Next

Abstract

In experiments on the cutaneothoracic muscle of the frog, we recorded, using the technique of two-electrode voltage clamp at a normal Ca^2+ concentration (1.8 mM), multiquantum end-plate currents (EPC) and miniature uniquantum EPC (mEPC). Multiquantum signals, when compared with uniquantum currents, were characterized by longer leading and trailing edges. The quantum composition of multiquantum signals estimated according to the ratios of EPC and mEPC amplitudes was, on average, 27% lower than that calculated according to the ratios of their integral values (areas). These data demonstrate that stimulus-evoked transmitter secretion from the motor nerve endings is noticeably asynchronous. Based on the parameters of the experimental EPC and mEPC, we estimated the temporal course of evoked secretion using various techniques: spectral analysis, a system of linear equations, and Van der Kloot's method. Using convolution with uniquantum signals, we found that spectral analysis is the best technique for such estimation. Calculated parameters of the temporal course of secretion were the following: risetime 0.20 msec and decay time constant 0.33 msec. The respective distribution significantly differed from that of the synaptic delays of extracellularly recorded uniquantum EPC by longer durations (150-200%) of the leading and trailing edges. We hypothesize that these differences are related to the geometry of the junction and the temporal sequence of switching on of the active zones in the nerve ending upon their activation by spreading action potentials. Factors influencing the temporal course of evoked secretion of the transmitter in the junction under study (its asynchronicity, in particular) are discussed.