Conversion of oleic acid to 10-hydroxystearic acid by whole cells of Stenotrophomonas nitritireducens

Author： Kim Bi-Na   Yeom Soo-Jin   Oh Deok-Kun  

Publisher： Springer Publishing Company

ISSN： 0141-5492

Source： Biotechnology Letters, Vol.33, Iss.5, 2011-05, pp. : 993-997

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Abstract

The optimal reaction conditions for the conversion of oleic acid to 10-hydroxystearic acid by whole cells of Stenotrophomonas nitritireducens were: pH 7.5, 35°C, 0.05% (w/v) Tween 80, 20 g cells l−1, and 30 g oleic acid l−1 in an anaerobic atmosphere. Under these conditions, the cells produced 31.5 g 10-hydroxystearic acid l−1 over 4 h with a conversion yield of 100% (mol/mol) and a productivity of 7.9 g l−1 h−1, indicating that oleic acid was converted completely to 10-hydroxystearic acid, with no detectable byproduct. This is the highest concentration, productivity, and yield of 10-hydroxystearic acid from oleic acid reported thus far.

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