

Author: García Noemí Correa Francisco Chávez Edmundo
Publisher: Springer Publishing Company
ISSN: 0145-479X
Source: Journal of Bioenergetics and Biomembranes, Vol.37, Iss.1, 2005-02, pp. : 17-23
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Abstract
In this work we studied permeability transition by incubating mitochondria in the presence of 50 μM Ca2+ and malate/glutamate as substrates. This condition, besides inducing the release of pyridine nucleotides, promotes the generation of reactive oxygen-derived species by the complex I of the respiratory chain. The latter leads to the opening of the mitochondrial permeability transition pore. Ca2+ release, mitochondrial swelling and collapse of the transmembrane electric potential, were analyzed to assess this process. We propose that the mechanism for pore opening, in addition to the oxidative stress, involves the uncoupling effect of fatty acids providing activation of phospholipase A2, lipid peroxidation, and the oxidation of membrane thiols. This proposal emerges from the data indicating the protective effect of bovine serum albumin and
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