Somatic embryogenesis and organogenesis in Dendrocalamus hamiltonii

Author: Zhang Ning   Fang Wei   Shi Yan   Liu Qianqian   Yang Haiyun   Gui Renyi   Lin Xinchun  

Publisher: Springer Publishing Company

ISSN: 0167-6857

Source: Plant Cell, Tissue and Organ Culture, Vol.103, Iss.3, 2010-12, pp. : 325-332

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Abstract

In this study, mature zygotic embryos, plant growth regulators, and various media were tested with the aim of developing an efficient regeneration system for plantlets of the bamboo species Dendrocalamus hamiltonii. Callus formation was induced in explants cultured in Murashige and Skoog (MS) medium supplemented with 1.0–3.0 mg/l 2,4-dichlorophenoxyacetic acid. Optimal shoot differentiation and subsequent shoot growth were also obtained in MS medium supplemented with 2 mg/l benzyladenine, 1 mg/l kinetin, and 1 mg/l naphthaleneacetic acid. Root induction was enhanced by the addition of 5 mg/l indole-3-butyric acid to the culture medium. Histological analysis revealed that both somatic embryogenesis and organogenesis were induced during callus initiation, shoot differentiation, and the development of plantlets from the mature zygotic embryos. Our data provide a useful basis for developing culture protocols for the regeneration of bamboo plants.

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