Transformation and Plant Regeneration from Leaf Explants of Solanum tuberosum L. cv. ‘Shepody’

Author： Gustafson V. Mallubhotla S. MacDonnell J. Sanyal-Bagchi M. Chakravarty B. Wang-Pruski G. Rothwell C. Audy P. Koeyer D. Siahbazi M Flinn B. Regan S.

Publisher： Springer Publishing Company

ISSN： 0167-6857

Source： Plant Cell, Tissue and Organ Culture, Vol.85, Iss.3, 2006-06, pp. : 361-366

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Abstract

As part of a large-scale genomics project focused on understanding and improving the Shepody potato, we have increased the regeneration and transformation rates for this cultivar. Using combinations of auxins and trans-zeatin, leaf and stem explants were evaluated for callus induction and shoot formation. Several plant growth regulator combinations resulted in higher plant regeneration rates over a previous method. Using the best combination of auxin and cytokinin in combination with Agrobacterium-mediated transformation, we regenerated independent putative transformants from 59.5% of the total explants plated. We ran PCR on a sample of the plants to confirm transformation and 47.1% were nptII positive; giving a confirmed transformation rate of 28.0%.