Author: Gaietta Guido M. Yoder Elizabeth J. Deerinck Tom Kinder Karen Hanono Abraham Han Areum Wu Chun Ellisman Mark H.
Publisher: Springer Publishing Company
ISSN: 0300-4864
Source: Journal of Neurocytology, Vol.32, Iss.4, 2003-05, pp. : 373-380
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Abstract
Pharmacological approaches and optical recordings have shown that Schwann cells of a myelinating phenotype are activated by 5-HT upon its interaction with the 5-HT2A receptor (5-HT2AR). In order to further characterize the expression and distribution of this receptor in Schwann cells, we examined rat sciatic nerve and cultured rat Schwann cells using probes specific to 5-HT2AR protein mRNA. We also examined the endogenous sources of 5-HT in rat sciatic nerve by employing both histochemical stains and an antibody that specifically recognizes 5-HT. Rat Schwann cells of a myelinating phenotype contained both 5-HT2AR protein and mRNA. In the healthy adult rat sciatic nerve, 5-HT2ARs were evenly distributed along the outermost portion of the Schwann cell plasma membrane and within the cytoplasm. The most prominent source of 5-HT was within granules of the endoneurial mast cells, closely juxtaposed to Schwann cells within myelinating sciatic nerves. These results support the hypothesis that the 5-HT receptors expressed by rat Schwann cells
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