Author: Gao Chun-Lin Liu Guang-Ling Liu Shi Chen Xiao-Hui Ji Chen-Bo Zhang Chun-Mei Xia Zheng-Kun Guo Xi-rong
Publisher: Springer Publishing Company
ISSN: 0300-8177
Source: Molecular and Cellular Biochemistry, Vol.353, Iss.1-2, 2011-07, pp. : 215-223
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
The co-transcription factor peroxisome proliferator-activated receptor γ coactivator-1β (PGC-1β) was first identified in 2002. Although the function of PGC-1β in white adipose tissue (WAT) is largely unknown, it has been studied extensively in the liver, cardiac muscle, and skeletal muscle. Herein, we investigated PGC-1β overexpression in 3T3-L1 adipocytes. The main findings were as follows: (i) 3T3-L1 adipocytes overexpressing PGC-1β showed improved insulin sensitivity and elevated insulin-stimulated glucose uptake; (ii) mitochondrial cristae became broader and more ordered, additional smaller mitochondria emerged, mitochondrial DNA increased, and fission 1 protein (Fis1) mRNA expression was greatly elevated; (iii) intracellular ATP levels increased, but no changes were observed in mitochondrial membrane potential, uncoupling protein (UCP) mRNA expression, or reactive oxygen species (ROS) production; and (iv) mitochondrial metabolism factors, namely, acetyl-coenzyme A carboxylase 2 (ACC2) and hexokinase 2 (HK2) were downregulated, while cytochrome c oxidase subunit IV (COX IV) was upregulated. In conclusion, PGC-1β affects not only insulin sensitivity but also mitochondrial biogenesis and function. We believe that the role of PGC-1β is distinct from that of PGC-1α in WAT.
Related content
By Zhu Shunming Sun Fei Li Weijie Cao Yanjie Wang Chen Wang Yabin Liang Dong Zhang Rongqing Zhang Shenwei Wang Haichang Cao Feng
Molecular and Cellular Biochemistry, Vol. 353, Iss. 1-2, 2011-07 ,pp. :