Ornithine Decarboxylase Activity in Cerebral Post-Ischemic Reperfusion Damage: Effect of Methionine Sulfoximine

Author: Di Giacomo C.   Sorrenti V.   Acquaviva R.   Campisi A.   Vanella G.   Perez-Polo J. R.   Vanella A.  

Publisher: Springer Publishing Company

ISSN: 0364-3190

Source: Neurochemical Research, Vol.22, Iss.9, 1997-09, pp. : 1145-1150

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Abstract

Excessive activation of glutamate receptors via the N-methyl-D-aspartate (NMDA) subtype appears to play a role in the sequence of cellular events which lead to irreversible ischemic damage to neurons. Furthermore, NMDA receptor activation induces a stimulation of ornithine decarboxylase (ODC), the rate-limiting enzyme for polyamine (PA) biosynthesis. In order to better understand the role of PA we have measured ODC activity and the effect of methionine sulfoximine (MSO), a molecule able to stimulate ODC, on a model of transient cerebral ischemia. There was a significant increase in ODC activity in the rat cerebral cortex during post-ischemic reperfusion. The treatment with MSO induced a significant decrease in cerebral glutamine synthetase activity accompained by a marked increase in ODC activity. In MSO-pretreated rats there was a significant decrease in the survival rate when compared to untreated ischemic rats.

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