Author: Li Yu-Teh Li Su-Chen Buck Wayne Haskins Mark Wu Sz-Wei Khoo Kay-Hooi Sidransky Ellen Bunnell Bruce
Publisher: Springer Publishing Company
ISSN: 0364-3190
Source: Neurochemical Research, Vol.36, Iss.9, 2011-09, pp. : 1612-1622
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
To facilitate the study of the chemical pathology of galactosylsphingosine (psychosine, GalSph) in Krabbe disease and glucosylsphingosine (GlcSph) in Gaucher disease, we have devised a facile method for the effective separation of these two glycosylsphingosines from other glycosphingolipids (GSLs) in Krabbe brain and Gaucher spleen samples. The procedure involves the use of acetone to selectively extract GalSph and GlcSph, respectively, from Krabbe brain and Gaucher spleen samples. Since acetone does not extract other GSLs except modest amounts of galactosylceramide, sulfatide, and glucosylceramide, the positively charged GalSph or GlcSph in the acetone extract can be readily separated from other GSLs by batchwise cation-exchange chromatography using a Waters Accell Plus CM Cartridge. GalSph or GlcSph enriched by this simple procedure can be readily analyzed by thin-layer chromatography or high-performance liquid chromatography.
Related content
Acetylated tau, a novel pathological signature in Alzheimer's disease and other tauopathies
Brain, Vol. 135, Iss. 3, 2012-04 ,pp. :