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Production of channel catfish (Ictalurus punctatus) recombinant gonadotropins using the S2 Drosophila cell line system

Author： Zmora N. Kumar S. Kazeto Y. Trant J.M.

Publisher： Springer Publishing Company

ISSN： 0920-1742

Source： Fish Physiology and Biochemistry, Vol.28, Iss.1-4, 2003-01, pp. : 475-477

Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.

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Abstract

The S2 cell system was utilized for the production of recombinant luteinizing hormone (LH) and follicle stimulating hormone (FSH) of the channel catfish (Ictalurus punctatus) as C-terminal His-tagged proteins. When expressed individually, the common -subunit was secreted in abundance but both &bgr;-LH and &bgr;-FSH were poorly expressed. However, co-expression of the -subunit with each of the &bgr; subunits using a duel promoter vector resulted in the abundant secretion of LH and FSH /&bgr; heterodimers. These recombinant gonadotropins (GtH) were able to stimulate estradiol secretion in an ovarian follicle bioassay and activate recombinant gonadotropin receptors.

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