Identification and in planta detection of Pseudomonas syringae pv. tomato using PCR amplification of hrpZPst

Author： Zaccardelli Massimo   Spasiano Annalisa   Bazzi Carlo   Merighi Massimo  

Publisher： Springer Publishing Company

ISSN： 0929-1873

Source： European Journal of Plant Pathology, Vol.111, Iss.1, 2005-01, pp. : 85-90

Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.

Previous Menu Next

Abstract

A rapid detection method based on PCR amplification of Pseudomonas syringae pv. tomato chromosomal sequences was developed. Primer design was based on the P. syringae DC3000 hrpZPst gene, which maps on a pathogenicity-associated operon of the hrp/hrc pathogenicity island.A 532 bp product corresponding to an internal fragment of hrpZPst was amplified from 50 isolates of P. syringae pv. tomato belonging to a geographically representative collection. The amplification product was also obtained from three coronatine-deficient strains of P. syringae pv. tomato.On the other hand, PCR did not produce any such products from 100 pathogenic and symbiotic bacterial strains of the genera Pseudomonas, Xanthomonas, Erwinia, and Rhizobium and 75 unidentified bacterial saprophytes isolated from tomato plants. The method was tested using leaf and fruit spots from naturally-infected tomato plants and asymptomatic nursery plants and artificially contaminated tomato seeds. The results confirmed the high specificity observed using pure cultures.

Related content

The Use of Bioluminescence for Monitoring in planta Growth Dynamics of a Pseudomonas syringae Plant Pathogen

By Paynter Christopher   Salisbury Vyvyan   Arnold Dawn   Jackson Robert  

European Journal of Plant Pathology, Vol. 115, Iss. 3, 2006-07 ,pp. : 363-366 (4)

Springer Publishing Company

Access to resources Recommend Favorite

Biocontrol activity of Bacillus against a GFP-marked Pseudomonas syringae pv. tomato on tomato phylloplane

By Lanna Filho R.   Souza R.   Ferreira A.   Quecine M.   Alves Eduardo   Azevedo J.  

Australasian Plant Pathology, Vol. 42, Iss. 6, 2013-11 ,pp. : 643-651 (9)

Springer Publishing Company

Access to resources Recommend Favorite

Extracts of the marine brown macroalga, Ascophyllum nodosum, induce jasmonic acid dependent systemic resistance in Arabidopsis thaliana against Pseudomonas syringae pv. tomato DC3000 and Sclerotinia sclerotiorum

By Subramanian Sowmyalakshmi   Sangha Jatinder   Gray Bruce   Singh Rudra   Hiltz David   Critchley Alan   Prithiviraj Balakrishnan  

European Journal of Plant Pathology, Vol. 131, Iss. 2, 2011-10 ,pp. : 237-248 (12)

Springer Publishing Company

Access to resources Recommend Favorite

In vitro culture response of common bean explants to filtrate from Pseudomonas syringae pv. Phaseolicola and correlation with disease resistance

By González A.   Polanco C.   Ruiz M.  

In Vitro Cellular and Development Biology - Plant, Vol. 42, Iss. 2, 2006-03 ,pp. : 160-164 (5)

Springer Publishing Company

Access to resources Recommend Favorite

Detection and identification of the crucifer pathogen, Xanthomonas campestris pv. campestris, by PCR amplification of the conserved Hrp/type III secretion system gene hrcC

By Zaccardelli Massimo   Campanile Francesco   Spasiano Annalisa   Merighi Massimo  

European Journal of Plant Pathology, Vol. 118, Iss. 3, 2007-07 ,pp. : 299-306 (8)

Springer Publishing Company

Access to resources Recommend Favorite