Short communication: Satellite DNA-based species-specific identification of single individuals of the pinewood nematode Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae)

Author： Castagnone Chantal   Abad Pierre   Castagnone-Sereno Philippe  

Publisher： Springer Publishing Company

ISSN： 0929-1873

Source： European Journal of Plant Pathology, Vol.112, Iss.2, 2005-06, pp. : 191-193

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Abstract

The pinewood nematode Bursaphelenchus xylophilus is a severe pest of coniferous trees, and has been designated as a quarantine organism in the European Union. From the sequence of a satellite DNA family characterized in the genome of this nematode, we developed a PCR procedure that allowed the specific discrimination of this species from closely related Bursaphelenchus species found on coniferous trees. Moreover, because of the repetitive nature of satellite DNA, positive amplification was achieved from B. xylophilus single individuals, which should contribute to an easy diagnostic procedure for assisting in the management of this major pest of conifer forests.

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