Optimization of fermentation conditions for production of xylanase by a newly isolated strain, Penicillium thiersii ZH-19

Author： Cui Fengjie   Li Yin   Liu Zhiqiang   Zhao Hui   Ping Lifeng   Ping Liying   Yang Yinan   Xue Yaping   Yan Lijiao  

Publisher： Springer Publishing Company

ISSN： 0959-3993

Source： World Journal of Microbiology and Biotechnology, Vol.25, Iss.4, 2009-04, pp. : 721-725

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Abstract

The objective of this study was to maximize production of xylanase by a newly isolated strain Penicillium thiersii ZH-19. Response surface methodology was employed to study the effects of significant factors such as pH, temperature, xylan concentration, and cultivation time, on the production of xylanase by Penicillium thiersii ZH-19. The optimal fermentation parameters for enhanced xylanase production were found to be pH 7.72, temperature 24.8°C, xylan 13.2 g l−1 and the fermentation time 125.8 h. The model predicted a xylanase activity of 75.24 U ml−1. Verification of the optimization showed that the maximum xylanase production reached 73.50 U mL−1 in the flask experiments and 80.23 U mL−1 in the scale of 15-L fermenter under the optimal condition.

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