Identification of the aph IV gene from protoplast-derived maize plants by a simple nonradioactive DNA hybridization method

Author: Hill Martha   Melanson Denise   Wright Martha  

Publisher: Springer Publishing Company

ISSN: 1475-2689

Source: In Vitro Cellular and Development Biology - Plant, Vol.35, Iss.2, 1999-03, pp. : 154-155

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Abstract

Protoplast-derived, transformed maize plants were evaluated by Southern analysis for the presence of the aph IV gene which codes for resistance to the antibiotic, hygromycin B. This gene was used as a selectable marker for the transformation of maize protoplasts. Southern analysis was performed with fluorescein-labeled probe DNA. A new method for labeling molecular weight markers with fluorescein-N6 is presented. The nonradioactive Southern analysis method is compared to the radioactive method and the results show that the nonradioactive method is as sensitive as the radioactive method.

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