Influence of some adjuvants on in vitro clonal propagation of male and female jojoba plants

Author： Prakash Surya   Agrawal Veena   Gupta S.  

Publisher： Springer Publishing Company

ISSN： 1475-2689

Source： In Vitro Cellular and Development Biology - Plant, Vol.39, Iss.2, 2003-03, pp. : 217-222

Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.

Previous Menu Next

Abstract

The influence of different adjuvants, activated charcoal (AC), casein hydrolyzate (CH), coconut water (CW), polyvinylpyrrolidone (PVP), and triiodobenzoic acid (TIBA), has been assessed on the shoot production potential of the nodal explants derived from in vitro-raised male and female jojoba (Simmondsia chinensis) shoots. Nodal explants of each sex were cultured separately on Murashige and Skoog medium supplemented with different levels of AC, CW, CH, PVP, and TIBA either alone or along with optimum levels of N 6-benzyladenine (BA; 10 μM for male, 20 μM for female). Some differences in response of the explants of both the sexes have been observed in terms of (1) percentage of explants developing shoots, (2) average shoot number, and (3) average shoot length. AC alone proved beneficial for elevating morphogenic response in male as well as female explants in comparison to basal medium or media containing AC and the optimum level of BA. When used alone, CH proved inhibitory for shoot differentiation in both sexes, especially in male explants. Addition of PVP to MS enhanced shoot proliferation in female explants only, but along with BA it increased the response of male explants. BA in combination with different levels of TIBA promoted shoot multiplication in female explants. Thus, explants of both male and female shoots exhibited differential morphogenic behavior under the influence of various adjuvants. However, BA alone proved to be the best for differentiation of shoots in both male (10 μM) as well as female (20 μM) explants.

Related content

In vitro propagation of some important chinese medicinal plants and their sustainable usage

By Nalawade Satish   Tsay Hsin-Sheng  

In Vitro Cellular and Development Biology - Plant, Vol. 40, Iss. 2, 2004-03 ,pp. : 143-154 (12)

Springer Publishing Company

Access to resources Recommend Favorite

In vitro clonal propagation and regeneration of the commercially important plant Mexican oregano (Lippia graveolens)

By Castellanos-Hernández Osvaldo   Acevedo-Hernández Gustavo   Torres-Morán Martha   Zurita Florentina   Gutiérrez-Lomelí Melesio   Toro-Sánchez Carmen   Rodríguez-Sahagún Araceli  

In Vitro Cellular and Development Biology - Plant, Vol. 49, Iss. 5, 2013-11 ,pp. : 620-625 (6)

Springer Publishing Company

Access to resources Recommend Favorite

In vitro clonal propagation of annatto (Bixa oreliana L.)

By D'Souza Marie-Claire   Sharon Madhuri  

In Vitro Cellular and Development Biology - Plant, Vol. 37, Iss. 2, 2001-03 ,pp. : 168-172 (5)

Springer Publishing Company

Access to resources Recommend Favorite

Stimulation of root development with cyclodextrins on jojoba shoots in vitro

By Apóstolo Nancy   Brutti Cristina   Ferrarotti Susana   Llorente Berta   Krymkiewicz Norberto  

In Vitro Cellular and Development Biology - Plant, Vol. 37, Iss. 3, 2001-05 ,pp. : 414-418 (5)

Springer Publishing Company

Access to resources Recommend Favorite

Improvement of jojoba shoot multiplication in vitro by ventilation

By Mills David   Yanqing Zhou   Benzioni Aliza  

In Vitro Cellular and Development Biology - Plant, Vol. 40, Iss. 4, 2004-07 ,pp. : 396-402 (7)

Springer Publishing Company

Access to resources Recommend Favorite