Isolation and characterization of microsatellite markers from Hibiscus rosa-sinensis (Malvaceae) and cross-species amplifications

Author： Bruna Simona   Portis Ezio   Braglia Luca   Benedetti Laura   Comino Cinzia   Acquadro Alberto   Mercuri Antonio  

Publisher： Springer Publishing Company

ISSN： 1566-0621

Source： Conservation Genetics, Vol.10, Iss.3, 2009-06, pp. : 771-774

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Abstract

We report on the development of 10 microsatellite markers in Hibiscus rosa-sinensis (Hrs). Three markers were obtained from sequences available in GenBank and seven were isolated using a two-step ‘primer extension’ procedure, based on the microsatellite-AFLP (M-AFLP) technique. Polymorphism was explored in 21 Hrs genotypes representing the genetic variation within commercial varieties. Inter-specific amplification was assessed on 12 Hibiscus wild species. A total of 45 and 56 alleles (ranging from 1 to 10 for each locus) was amplified respectively from the 21 Hrs varieties and among the full Hibiscus spp. genotype set. Primers and conditions for polymerase chain reaction (PCR) amplification of the detected loci are reported.

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