Spectrophotometric Determination of Acyclovir and Ribavirin in Their Dosage Forms

Author： El-Din Mohie Khaled Sharaf El-Brashy Amina Mohammed Sheribah Zeinab Awad El-Gamal Rania Mohammed

ISSN： 1944-7922

Source： Journal of AOAC International, Vol.89, Iss.3, 2006-05, pp. : 631-641

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Abstract

Two simple, accurate, and reliable spectrophotometric methods have been developed for the determination of 2 antiviral drugs, acyclovir (ACV) and ribavirin (RBV), in their pharmaceutical formulations. These methods are based on oxidation of the 2 drugs with either cerium (IV) ammonium sulfate (Method A) or potassium persulfate (Method B). The products of oxidation in both methods are coupled with 3-methylbenzothiazolin 2-one hydrazone, producing a deep blue color with a maximum absorption wavelength at 630 nm. In Method A, the absorbanceconcentration plots were linear over the ranges of 550 and 1060 g/mL with detection limits of 0.18 g/mL (8 10⁷ M) and 0.63 g/mL (2.58 10⁶ M) for ACV and RBV, respectively. In Method B, the ranges were 545 and 2050 g/mL with detection limits of 0.11 g/mL (4.88 10⁷ M) and 1.40 g/mL (5.73 10⁶ M) for the 2 drugs, respectively. The molar absorptivities were 4.1 10³ and 3.65 10³ L/mol/cm in Method A and 5.03 10³ and 3.97 10³ L/mol/cm in Method B for the 2 drugs, respectively. The proposed methods were applied successfully for the determination of the 2 drugs in their pharmaceutical formulations. The percentage recoveries standard deviation were 99.57 0.86 and 100.82 0.46 for ACV; 99.41 1.08 and 100.35 1.03 for RBV. The results obtained were compared statistically with those given by official methods and showed no significant differences regarding accuracy and precision.