A Capillary Zone Electrophoretic Method for Simultaneous Determination of Seven Drugs in Pharmaceuticals and in Human Urine

Author： Solangi Amber R Bhanger Muhammad I Memon Saima Q Khuhawar Muhammad Y Mallah Arfana

Publisher： AOAC International

ISSN： 1944-7922

Source： Journal of AOAC International, Vol.92, Iss.5, 2009-10, pp. : 1382-1389

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Abstract

A simple, sensitive, and novel method has been developed and validated for the separation and simultaneous quantitation of seven structurally different drugspipemidic acid and ofloxacin quinolone antibiotics, pseudoephedrine decongestant, piroxicam anti-inflammatory, thiamin, pyridoxine, and cobalaminin a mixture by capillary zone electrophoresis. Factors affecting the separation were pH, concentration of buffer, and applied voltage. Separation was carried out in <9 min with a 50 mM sodium tetraborate buffer, pH 10, and an applied voltage of 30 kV in an uncoated silica capillary tube. The carrier electrolyte gave baseline separation with good resolution, reproducibility, and accuracy. Calibration plots were linear over at least three orders of magnitude of analyte concentrations, and the lower LODs were within the range of 15 g/mL. Detection was performed by UV absorbance at 230 nm. The method was validated for the analysis of drugs in pharmaceutical preparations and in urine samples with RSD of 0.52.4 and recovery of >99.