Author： Dhoka Madhura V Sandage Shakuntala J Dumbre Snehal C
Publisher： AOAC International
ISSN： 1944-7922
Source： Journal of AOAC International, Vol.93, Iss.2, 2010-04, pp. : 531-535
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
A simple, precise, accurate, and sensitive RP-HPLC method for simultaneous determination of cefixime trihydrate and dicloxacillin sodium in combined tablet dosage form was developed and validated. Chromatographic separation of the two drugs was performed on a Purospher BDS C18 column (25 cm 4.6 mm id, 5 m particle size). The mobile phase methanol0.01 M phosphate buffer (75 + 25, v/v), adjusted to pH 3 with glacial acetic acid, was delivered at a flow rate of 1.0 mL/min. Detection was performed at 227 nm. Separation was completed within 10 min. Calibration curves were linear with R2 between 0.99 to 1.0 over a concentration range of 210 g/mL for cefixime trihydrate and 525 /mL for dicloxacillin sodium. The RSD for intraday and interday precision was <2.0.
Related content
By Sabnis Shweta Sadanand Dhavale Nilesh Dnvandev Jadhav Vijay Yeshawantrao Gandhi Santosh Vilashchand
Journal of AOAC International, Vol. 91, Iss. 2, 2008-02 ,pp. :
Access to resources
Recommend
By Gandhi Santosh Vilashchand Dhavale Nilesh Dnyandev Jadhav Vijay Yeshawantrao Sabnis Shweta Sadanand
Journal of AOAC International, Vol. 91, Iss. 1, 2008-01 ,pp. :
By Soni Hiral Kothari Charmy Khatri Deepak Mehta Priti
Journal of AOAC International, Vol. 97, Iss. 3, 2014-05 ,pp. :
By Martinc Botjan Grabnar Iztok Mrhar Ale Vovk Toma
Journal of AOAC International, Vol. 93, Iss. 4, 2010-07 ,pp. :