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Author: Kiryanov G. I. Kintsurashvili L. N. Isaeva L. V. Zakharova M. G.
Publisher: MAIK Nauka/Interperiodica
ISSN: 0006-2979
Source: Biochemistry (Moscow), Vol.69, Iss.9, 2004-09, pp. : 1044-1050
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Abstract
It has earlier been shown that multiple positioning of nucleosomes on mouse satellite DNA is determined by its nucleotide sequence. To clarify whether other factors, such as boundary ones, can affect the positionings, we modified the environment of satellite DNA monomer by inserting it into a yeast plasmid between inducible GalCyc promoter and a structural region of the yeast FLP gene. We have revealed that the positions of nucleosomes on satellite DNA are identical to those detected upon reconstruction
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