Bioelectrocatalysis of Dopamine Using Adsorbed Tyrosinase on Single-Walled Carbon Nanotubes

Author： Moghaddam Abdolmajid Bayandori Ganjali Mohammad Reza Niasari Marjan Ahadi Sara

ISSN： 0003-2719

Source： Analytical Letters, Vol.41, Iss.17, 2008-01, pp. : 3161-3176

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Abstract

A wealth of information on the reactions of redox-active sites in proteins can be obtained by voltammetric studies in which the protein sample is arranged as a layer on a suitable electrode surface. Here, we describe a method for the performance of a tyrosinase/single-walled carbon nanotubes/glassy carbon (Tyr/SWCNTs/GC) electrode, prepared by the modification of GC electrode surface by SWCNTs and adsorption of tyrosinase on the SWCNT surfaces. SWCNTs were studied with the help of scanning electron microscopy (SEM), transmission electron microscopy (TEM), and atomic force microscopy (AFM). The dimensions of SWCNTs make them ideal candidates for the adsorption of proteins. The copper-containing enzyme, tyrosinase, exhibited an electrical contact with the electrode, because of the structural alignment of the enzyme on the SWCNT surfaces. The apparent Michaelis-Menten constant (Km) for dopamine (DA) and the stability of the enzyme electrode were estimated. This method could be suitable for applications to nanofabricated devices.