Author： Lin Jiann-Tsyh
Publisher： Taylor & Francis Ltd
ISSN： 1082-6076
Source： Journal of Liquid Chromatography & Related Technologies, Vol.30, Iss.14, 2007-01, pp. : 2005-2020
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Abstract
Identification of complex acyl lipids ideally includes the normal-phase HPLC to separate the acyl lipid classes followed by reversed-phase HPLC to separate the molecular species of a lipid class. Both polar lipid classes and non-polar lipid classes have been separated by normal-phase HPLC, mostly on silica and diol columns using binary gradients. The tentative identification of lipid classes can be made by HPLC retention times and the co-chromatography with lipid class standards. Various normal-phase HPLC systems separating lipid classes are reviewed here.
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