Author： Wei Yun Ito Yoichiro
Publisher： Taylor & Francis Ltd
ISSN： 1082-6076
Source： Journal of Liquid Chromatography & Related Technologies, Vol.30, Iss.9-10, 2007-01, pp. : 1465-1473
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Abstract
Preparative high-speed countercurrent chromatography was successfully used for isolation and purification of hyperoside and luteolin-glucoside from Agrimonia pilosa Ledeb. The separation was performed by stepwise elution with a pair of two-phase solvent systems composed of ethyl acetate-methanol-water at volume ratios of 50:1:50 and 5:1:5, which had been selected by analytical high-speed countercurrent chromatography (HSCCC). Using a preparative unit of the HSCCC centrifuge, about a 300 mg amount of the crude extract was separated, yielding 7.3 mg of hyperoside and 10.4 mg of luteolin-glucoside at a high purity of over 97%.
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