Author： Cornelio Rafael Pavei Cabral Verza Simone Silva Ana Paula Ortega George Luzia Alisson Fialho Silvia Mayorga Paulo
Publisher： Taylor & Francis Ltd
ISSN： 1082-6076
Source： Journal of Liquid Chromatography & Related Technologies, Vol.32, Iss.19, 2009-01, pp. : 2857-2865
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
A fast and sensitive high performance chromatographic method for quantification of sodium alendronate was developed and afterward implemented for tablet content and dissolution test. Sodium alendronate reference standard and samples of two different commercial tablets were used. The analysis was performed using an anion exchange column at 30°C and a mixture of nitric acid 1.5 mM and copper II nitrate 0.5 mM (50:50, v/v) as the mobile phase. The analysis run time was 15 minutes and the detection was measured at 240 nm. The limit of detection was approximately 0.3 µg mL-1 and the limit of quantification was 0.9 µg mL-1. The within day and between day precision were 0.64 and 1.52%, respectively. The accuracy results showed a recovery range from 99.87 to 101.07%. The validated method was applied to the tablets content and tablet dissolution assays successfully.
Related content
Access to resources
Recommend
By Nilsson Carina Nilsson Frida Turner Pernilla Sixtensson Martin Nordberg Karlsson Eva Holst Olle Cohen Arieh Gorton Lo
Analytical and Bioanalytical Chemistry, Vol. 385, Iss. 8, 2006-08 ,pp. :
By Ding Jie Ren Nanqi Chen Ligang Wang Hui Ding Lan
Analytical Letters, Vol. 43, Iss. 5, 2010-01 ,pp. :
On-line preconcentration of phosphate onto molybdate form anion exchange column
By Taniai T. Sukegawa M. Sakuragawa A. Uzawa A.
Talanta, Vol. 61, Iss. 6, 2003-12 ,pp. :