Development of Thick and Highly Cell-Incorporated Engineered Tissues by Hydrogel Template Approach with Basic Fibroblast Growth Factor or Ascorbic Acid

Author： Yoshida Hiroaki Matsusaki Michiya Akashi Mitsuru

Publisher： Taylor & Francis Ltd

ISSN： 1568-5624

Source： Journal of Biomaterials Science, Polymer Edition, Vol.21, Iss.4, 2010-03, pp. : 415-428

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Abstract

We have previously reported a hydrogel template approach for the construction of centimeter-sized three-dimensional (3D) engineered tissues composed of cultured cells and extracellular matrices (ECM) produced by the cells. However, the interior of the engineered tissues was low in cell density; thus, it was pouched and non-dense morphologies. In this study, we developed thick and highly cell-incorporated engineered tissues by using basic fibroblast growth factor (bFGF) and ascorbic acid 2-phosphate (Asc 2-P). bFGF was loaded into freeze-dried poly(γ-glutamic acid) hydrogels with disulfide cross-links (γ-PGA-SS gels) as a template to enhance cell growth. After prescribed times of cell culture, the scaffolds were decomposed by adding a biocompatible reductant, cysteine, and cell proliferation and collagen production were investigated. The loading of bFGF into the scaffolds enhanced cellular invasion and proliferation. Meanwhile, the addition of Asc 2-P to the culture medium induced collagen production from the adhered fibroblasts. However, Asc 2-P did not affect cellular invasion into the template γ-PGA-SS gels. The volume and weight of the obtained tissues using bFGF after 28 days of culture were 1.3- and 1.4-fold higher than those of control tissues. The thick and highly cell-incorporated 3D-engineered tissues can be useful as a novel cell implantation material for tissue engineering.