Histone deacetylase inhibitor suberoyl bis-hydroxamic acid suppresses cell proliferation and induces apoptosis in breast cancer cells

Author：

Publisher： Spandidos Publications

E-ISSN： 1791-3004|11|4|2908-2912

ISSN： 1791-2997

Source： Molecular Medicine Reports, Vol.11, Iss.4, 2015-01, pp. : 2908-2912

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Abstract

Suberoyl bishydroxamic acid (SBHA) is a histone deacetylase inhibitor that has shown anticancer activity against numerous types of human cancer. The aim of the current study was to explore the effects of SBHA on the proliferation and apoptosis of breast cancer cells. MCF7 breast cancer cells were treated with different concentrations of SBHA and tested for cell viability, apoptosis and gene expression changes. The results showed that SBHA significantly inhibited the proliferation of MCF7 cells in a concentrationdependent manner, as determined using a Cell Counting kit8 assay. SBHAtreated MCF7 cells showed G0/G1 cellcycle arrest, coupled with elevated expression levels of p21 and p27 proteins. Hoechst 33258 staining revealed cell shrinkage, chromosomal condensation and nuclear fragmentation in MCF7 cells treated with SBHA. Flow cytometric analysis of Annexin Vstained cells showed that SBHA treatment induced apoptotic cell death in a concentrationdependent manner. Western blot analysis confirmed the upregulation of Bax and the downregulation of Bcl2 by SBHA. In conclusion, these results indicate that SBHA exerts cytotoxic effects against human breast cancer cells, which involves the modulation of p21, p27 and Bcl2 family proteins, consequently leading to cellcycle arrest and apoptosis.