Inhibition of the autophagy flux by gingerol enhances TRAIL-induced tumor cell death

Author： Nazim Uddin Md. Jeong Jae-Kyo Seol Jae-Won Hur Jin Eo Seong-Kug Lee John-Hwa Park Sang-Youel

E-ISSN： 1791-2431|33|5|2331-2336

ISSN： 1021-335X

Source： Oncology Reports, Vol.33, Iss.5, 2015-05, pp. : 2331-2336

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Abstract

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a primary anticancer agent and a member of the tumor necrosis factor family that selectively induces apoptosis in various tumor cells, but not in normal cells. Gingerol is a major ginger component with anti-inflammatory and antitumorigenic activities. Autophagy flux is the complete process of autophagy, in which the autophagosomes are lysed by lysosomes. The role of autophagy in cell death or cell survival is controversial. A549 adenocarcinoma cells are TRAIL-resistant. In the present study, we showed that treatment with TRAIL slightly induced cell death, but gingerol treatment enhanced the TRAIL-induced cell death in human lung cancer cells. The combination of gingerol and TRAIL increased accumulation of microtubule-associated protein light chain 3-II and p62, confirming the inhibited autophagy flux. Collectively, our results suggest that gingerol sensitizes human lung cancer cells to TRAIL-induced apoptosis by inhibiting the autophagy flux.