OphiopogoninD suppresses MDAMB435 cell adhesion and invasion by inhibiting matrix metalloproteinase9

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E-ISSN： 1791-3004|12|1|1493-1498

ISSN： 1791-2997

Source： Molecular Medicine Reports, Vol.12, Iss.1, 2015-01, pp. : 1493-1498

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Abstract

OphiopogoninD is one of steroidal saponins isolated from the root of the Chinese medicinal plant Ophiopogon japonicas. It has been claimed to possess antiinflammatory and antioxidant properties. The present study was the first to examine the antitumor metastasis properties of ophiopogoninD. An MTT assay showed that ophiopogoninD inhibited the proliferation of MDAMB435 melanoma cells, and decreased invasion was demonstrated using a Transwell invasion assay. Furthermore, adhesion of MDAMB435 cells to human umbilical vascular endothelial cells and to fibronectin was inhibited by ophiopogoninD. Gelatin zymography and western blot analysis showed that ophiopogoninD inhibited the expression and secretion of matrix metalloproteinase9 (MMP9), but not that of MMP2. Inhibition of phosphorylation of p38 by ophiopogoninD indicated its inhibition of the mitogenactivated protein kinase pathway. Overall, the results suggested that ophiopogoninD may be considered as a candidate drug for treating or preventing tumor metastasis.