HOTAIR is a potential target for the treatment of cisplatinresistant ovarian cancer

Author： Wang Yu Wang Hongli Song Tiefang Zou Yiting Jiang Jing Fang Lei Li Peiling

E-ISSN： 1791-3004|12|2|2211-2216

ISSN： 1791-2997

Source： Molecular Medicine Reports, Vol.12, Iss.2, 2015-08, pp. : 2211-2216

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Abstract

Previous studies have demonstrated that the presence of Hox transcript antisense intergenic RNA (HOTAIR) is correlated with poor survival in several types of cancer, including breast cancer, and promotes tumor metastasis. Currently, little is known regarding the correlation between HOTAIR and chemoresistance in cancer. The current study aimed to investigate the role of HOTAIR in epithelial ovarian cancer, and the correlation between HOTAIR and cisplatin resistance. Reverse transcription-quantitative polymerase chain reaction was conducted to detect HOTAIR expression in the ovarian specimens and ovarian carcinoma cell lines. The results indicated that the expression level of HOTAIR was higher in epithelial ovarian cancer tissues than the level in the benign ovarian tissues. The expression level was also higher in latestage malignant ovarian tumors compared with the level in earlystage tumors. Levels of HOTAIR were also higher in the SKOV3CDDP/R cisplatinresistant ovarian carcinoma cell line than in the SKOV3 cisplatinsensitive cell line. The knockdown of HOTAIR using siRNAs with transfection reagent suppressed cell proliferation, reduced the invasion ability of the cells and notably, it restored cisplatinsensitivity of the cisplatinresistant cells specifically by enhancing cisplatininduced cytotoxicity and apoptosis in SKOV3CDDP/R cells. In conclusion, HOTAIR may be used in the development of novel treatments for ovarian cancer, particularly those that are resistant to conventional therapies.