Transforming growth factor-β inhibits IQ motif containing guanosine triphosphatase activating protein 1 expression in lung fibroblasts via the nuclear factor-κB signaling pathway

Author：

Publisher： Spandidos Publications

E-ISSN： 1791-3004|12|1|442-448

ISSN： 1791-2997

Source： Molecular Medicine Reports, Vol.12, Iss.1, 2015-01, pp. : 442-448

Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.

Previous Menu Next

Abstract

IQ motif containing guanosine triphosphatase activating protein 1 (IQGAP1) is associated with idiopathic pulmonary fibrogenesis (IPF); however, characterization of the expression of IQGAP1 in lung fibroblasts has remained elusive. The present study therefore evaluated IQGAP1 expression in mouse and human lung fibroblasts under fibrotic conditions via western blot analysis. It was revealed that IQGAP1 expression levels were significantly decreased in lung fibroblasts isolated from bleomycinchallenged mice than in those of control mice. Transforming growth factorβ (TGFβ) induced differentiation, as well as decreased expression of IQGAP1 in WI38 cells human lung fibroblasts. Furthermore, inhibition of nuclear factor (NF)κB activation restored the TGFβinduced inhibition of IQGAP1 expression in WI38 cells. In lysophosphatidic acid (LPA)challenged WI38 cells, the expression of IQGAP1 was also decreased, while neutralized antiTGFβ antibody treatment restored the LPAinduced inhibition of IQGAP1 expression. These data indicated that TGFβ inhibited IQGAP1 expression in lung fibroblasts via the NFκB signaling pathway, presenting a potential novel therapeutic target for the treatment of IPF.