Publisher: Spandidos Publications
E-ISSN: 1792-1015|10|1|207-212
ISSN: 1792-0981
Source: Experimental and Therapeutic Medicine, Vol.10, Iss.1, 2015-01, pp. : 207-212
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Abstract
microRNAs (miRNAs) are involved in the regulation of almost all physiological and pathological processes, including osteoblast proliferation and differentiation. miR375 has been reported to be aberrantly expressed in various tumor types and to inhibit cell proliferation and invasion by targeting a number of key genes. However, the effects of miR375 on osteogenic differentiation have not been investigated. Thus, the present study aimed to elucidate the function served by miR375 in osteogenic differentiation. The expression levels of miR375 were observed to decrease in a C2C12 cell model of osteogenic differentiation. Overexpression of miR375 inhibited the activity of key osteoblast markers, including osteocalcin (OC), alkaline phosphatase (ALP) and collagen, type I, α 1 (COL1A1). By contrast, inhibition of miR375 expression resulted in an increase in the osteogenic potential, as indicated by the enhanced expression levels of OC, ALP and COL1A1. In addition, a dualluciferase reporter assay indicated that runtrelated transcription factor 2 (RUNX2) was a target of miR375. Western blot analysis revealed that the inhibition of miR375 led to a significant increase in the protein expression levels of RUNX2. In addition, overexpression of RUNX2 was observed to attenuate the miR375mediated suppression of osteogenic differentiation. Therefore, the results demonstrated that miR375 was able to inhibit osteogenic differentiation via the regulation of RUNX2 expression.
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