Identification of Target Antigens of Antiendothelial Cell Antibodies Against Human Brain Microvascular Endothelial Cells in Healthy Subjects

Publisher： Bentham Science Publishers

E-ISSN： 1875-5739|12|1|25-30

ISSN： 1567-2026

Source： Current Neurovascular Research, Vol.12, Iss.1, 2015-02, pp. : 25-30

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Abstract

Antiendothelial cell antibodies (AECAs) have been detected in patients who have autoimmuneand inflammatory diseases. Previous studies showed that AECAs against human umbilical vein endothelialcells were detected in healthy subjects. In the present study, we evaluated AECAs against human brainmicrovascular endothelial cells (HBMEC) in serum. We detected 250 antigen spots that reacted withAECAs in serum samples from 30 healthy subjects by 2-dimensional immunoblot using primary culturedHBMEC as the antigen source. There were 10 spots that corresponded to common target antigen spots andreacted with AECAs in serum samples from > 25% of the 30 healthy subjects. We identified 7 proteins that correspondedto 8 of the 10 spots by mass spectrometry: 78-kDa glucose-regulated protein, dihydropyrimidinase-related protein 2,heterogeneous nuclear ribonucleoprotein L, vimentin, perilipin 3, alpha-enolase, and annexin A2. The results suggest thatthese 7 HBMEC proteins are major target antigens of natural AECAs.